Transcriptomics

Dataset Information

240

Transcription profiling by array of Arabidopsis thaliana plants from the genotypes wild type and ABA2OE (35S:ABA2 overexpressor in Col-0 background) subjected to mild light stress


ABSTRACT: Arabidopsis thaliana plants from the genotypes Col-0 and ABA2OE (35S:ABA2 overexpressor in Col-0 background) were used. They were grown in a growth chamber for 5 weeks in short day (8/16 light/dark cycle) at 22 degrees, 150 umol*m2*s-1 (fluorescent lights) and 55% RH in 100-ml pots with Levington's L2+S. On the day of the experiment, two hours after the lights turned on they were subjected to mild light stress (approx. 1500 umol*m-2*s-1, measured with a PAR light meter) for 30 min under an Isolight machine (white LED array), and immediately snap-frozen in liquid nitrogen. The outer leaves from four biological replicates were ground and RNA purified, and labelled RNA was produced with the Agilent Quick Amp kit. Labelled RNA was hybridized to Agilent Arabidopsis V4 microarray slides, and the fluorescence recorded with a Genepix scanner.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Philip M Mullineaux   Ruben Alvarez-Fernandez  

PROVIDER: E-MTAB-2048 | ArrayExpress | 2014-02-10

REPOSITORIES: ArrayExpress

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Publications

Abscisic acid signalling determines susceptibility of bundle sheath cells to photoinhibition in high light-exposed Arabidopsis leaves.

Gorecka Magdalena M   Alvarez-Fernandez Ruben R   Slattery Katie K   McAusland Lorna L   Davey Phillip A PA   Karpinski Stanislaw S   Lawson Tracy T   Mullineaux Philip M PM  

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20140303 1640


The rapid induction of the bundle sheath cell (BSC)-specific expression of ASCORBATE PEROXIDASE2 (APX2) in high light (HL)-exposed leaves of Arabidopsis thaliana is, in part, regulated by the hormone abscisic acid (ABA) produced by vascular parenchyma cells. In this study, we provide more details of the ABA signalling that regulates APX2 expression and consider its importance in the photosynthetic responses of BSCs and whole leaves. This was done using a combination of analyses of gene expressio  ...[more]

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