Transcriptomics

Dataset Information

242

Methylation profiling by array of Danio rerio liver samples from F0, F1 and F2 embryos and MeDIP samples to determine DNA methylation changes following chemical exposure and whether these persisted transgenerationally


ABSTRACT: Adult F0 female zebrafish were fed with control diet or diets containing 5-AZA, MeHg or TCDD. After breeding with unexposed males to produce the F1 generation, livers were sampled from the F0 females. F1 generation embryos were unexposed to test chemicals, were sampled, then bred to produce F2 fish, also unexposed to test chemicals. Methylated DNA immunoprecipitation was carried out on liver samples from F0 and F1 and F2 embryos and MeDIP samples were labeled with Cy5 and hybridised to a zebrafish CGI tiling array versus Cy3-labled zebrafish genomic DNA. The objective was to determine DNA methylation changes following chemical exposure and whether these persisted transgenerationally.

ORGANISM(S): Danio rerio  

SUBMITTER: Tim D Williams  

PROVIDER: E-MTAB-2057 | ArrayExpress | 2014-03-01

REPOSITORIES: ArrayExpress

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Publications

Impacts of TCDD and MeHg on DNA methylation in zebrafish (Danio rerio) across two generations.

Olsvik Pål A PA   Williams Timothy D TD   Tung Hui-shan HS   Mirbahai Leda L   Sanden Monica M   Skjaerven Kaja H KH   Ellingsen Ståle S  

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 20140527


This study aimed to investigate whether dioxin (TCDD) and methylmercury (MeHg) pose a threat to offspring of fish exposed to elevated concentrations of these chemicals via epigenetic-based mechanisms. Adult female zebrafish were fed diets added either 20 μg/kg 2,3,7,8 TCDD or 10 mg/kg MeHg for 47 days, or 10 mg/kg 5-aza-2'-deoxycytidine (5-AZA), a hypomethylating agent, for 32 days, and bred with unexposed males in clean water to produce F1 and F2 offspring. Global DNA methylation, promoter CpG  ...[more]

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