Transcriptomics

Dataset Information

300

Ago1-bound short RNAs in HeLa-S3 cell line


ABSTRACT: Characterization of AGO1 bound short RNAs from nuclear extracts of HeLaS3 cell line. Nuclei were isolated from HeLaS3 cells; a fraction of Nuclear lysates was harvsted and sequenced as the input samples. The rest of nuclear lysates were immunoprecipitated using AGO1 monoclonal antibodies or Isotype matched IgG. Following immunoprecipitation, RNA was extracted from AGO1 or control IgG IP for short RNA sequencing. Two independent biological replicates were carried out.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens  

SUBMITTER: Valerio Fulci  

PROVIDER: E-MTAB-2129 | ArrayExpress | 2015-05-17

SECONDARY ACCESSION(S): ERP004389

REPOSITORIES: ArrayExpress, ENA

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Publications

ARGONAUTE2 cooperates with SWI/SNF complex to determine nucleosome occupancy at human Transcription Start Sites.

Carissimi Claudia C   Laudadio Ilaria I   Cipolletta Emanuela E   Gioiosa Silvia S   Mihailovich Marija M   Bonaldi Tiziana T   Macino Giuseppe G   Fulci Valerio V  

Nucleic acids research 20150120 3


Argonaute (AGO) proteins have a well-established role in post-transcriptional regulation of gene expression as key component of the RNA silencing pathways. Recent evidence involves AGO proteins in mammalian nuclear processes such as transcription and splicing, though the mechanistic aspects of AGO nuclear functions remain largely elusive. Here, by SILAC-based interaction proteomics, we identify the chromatin-remodelling complex SWI/SNF as a novel AGO2 interactor in human cells. Moreover, we show  ...[more]

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