Transcriptomics

Dataset Information

417

Parallel RNA-seq and ribosome profiling of meiotic fission yeast cells


ABSTRACT: We applied in parallel RNA-Seq and Ribosome-profiling to S. pombe pat1 diploids undergoing meiosis and sporulation in a synchronous manner

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Schizosaccharomyces pombe  

SUBMITTER: Juan Mata  

PROVIDER: E-MTAB-2265 | ArrayExpress | 2014-06-03

SECONDARY ACCESSION(S): ERP004657

REPOSITORIES: ArrayExpress, ENA

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Publications

The translational landscape of fission-yeast meiosis and sporulation.

Duncan Caia D S CD   Mata Juan J  

Nature structural & molecular biology 20140615 7


Sexual development in Schizosaccharomyces pombe culminates in meiosis and sporulation. We used ribosome profiling to investigate the translational landscape of this process. We show that the translation efficiency of hundreds of genes is regulated in complex patterns, often correlating with changes in RNA levels. Ribosome-protected fragments show a three-nucleotide periodicity that identifies translated sequences and their reading frame. Using this property, we identified 46 new translated genes  ...[more]

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