Immunosuppressive capabilities of mesenchymal stromal cells are maintained under hypoxic growth conditions and after gamma irradiation
ABSTRACT: To investigate factors that might influence the T-cell suppressive capabilities of mesenchymal stroma cells, T-cell suppressive and non-suppressive mesenchymal stroma cells were compared. Immunosuppressive capabilities of MSCs was determined via T-cell proliferation assay.
<h4>Background aims</h4>The discovery of regenerative and immunosuppressive capacities of mesenchymal stromal cells (MSCs) raises hope for patients with tissue-damaging or severe, treatment-refractory autoimmune disorders. We previously presented a method to expand human MSCs in a bioreactor under standardized Good Manufacturing Practice conditions. Now we characterized the impact of critical treatment conditions on MSCs with respect to immunosuppressive capabilities and proliferation.<h4>Method ...[more]
Project description:SW620 were transfected with siMYC or siCTR at time point 0h. 24h later cells were splitter 1:2, additional 24 h later (48h after transfection) cells were treated with BEZ235 (200nM) or DMSO. Cells were harvested after additional 24h (72h after siRNA transfection; 24h BEZ235 treatment.
Project description:HUWE1 was depleted in Ls174T cells with an shRNA and the expression changes relative to a control shRNA were analyzed with microarray. Ls174T cells were treated with two separate HUWE1 inhibitor compounds (BI8622/BI8626) at concentrations of 20 uM and expression profiles relative to DMSO treated control were monitored via microarray. HUWE1-depleted Ls174T cells were treated with BI8622 (20uM) and expression profiles relative to BI8622-treated non-depleted cells were analyzed.
Project description:Ascites-associated macrophages were collected from human ovarian carcinoma patients and their expression profiles determined via Agilent microarrays. In addition, monocyte derived macrophages from healthy donors were differentiated ex-vivo with MCSF and GMCSF and their expression profiles determined as well.
Project description:The effect of PPARbeta/delta agonists and inverse agonists on GM-CFS/IL-4 induced differentiation of bone marrow derived cells was characterized by transcriptional profiling via micro array.
Project description:Myc was depleted in HeLa cells (ATCC CRL-1934) with an shRNA and the resulting expression profile changes mapped via microarrays. This study is the equivalent of E-MTAB-1524 . E-MTAB-1524 has been replaced by E-MTAB-1886.
Project description:Pancreatic ductal adenocarcinoma (PDAC) cells undergo epithelial mesenchymal transdifferentiation (EMT) in adaption to environmental cues, including inflammation, a process that combines tumour cell dedifferentiation with dissemination and acquisition of stemness features. However, the mechanisms coupling inflammation-induced signalling pathways with EMT and stemness remain largely unknown. Here, we reveal the inflammation-induced transcription factor NFATc1 as a central regulator of pancreatic cancer cell plasticity.