Transcriptomics

Dataset Information

415

RNA-seq of total RNA from vegetatively growing fission yeast cells


ABSTRACT: We RNA-Seq to total RNA extracted from vegetatively growing S. pombe cells.

INSTRUMENT(S): Illumina NextSeq 500

ORGANISM(S): Schizosaccharomyces pombe  

SUBMITTER: Juan Mata  

PROVIDER: E-MTAB-2470 | ArrayExpress | 2014-06-03

SECONDARY ACCESSION(S): ERP005530

REPOSITORIES: ArrayExpress, ENA

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Publications

The translational landscape of fission-yeast meiosis and sporulation.

Duncan Caia D S CD   Mata Juan J  

Nature structural & molecular biology 20140615 7


Sexual development in Schizosaccharomyces pombe culminates in meiosis and sporulation. We used ribosome profiling to investigate the translational landscape of this process. We show that the translation efficiency of hundreds of genes is regulated in complex patterns, often correlating with changes in RNA levels. Ribosome-protected fragments show a three-nucleotide periodicity that identifies translated sequences and their reading frame. Using this property, we identified 46 new translated genes  ...[more]

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