Transcriptomics

Dataset Information

64

Transcription profiling of pigment cells in the tunicate Ciona intestinalis to study pigment cell specification within Central Nervous System


ABSTRACT: Its known that FGF signaling induces pigment cell precursors formation. We perturbed the endogenous FGF signaling cascade by using a dominant-negative form of the unique Ci-FGF receptor (FGFRDN) and a constitutively active form of Ci-Ets1/2 (Ets:Vp16), a transcriptional effector of FGF/MAP Kinase cascade. Targeted expression of dominant negative FGF receptor and Ets:VP16 fusion protein was achieved in PCPs using the Tyrosinase-related protein 1/2a (Tyrp1/2a) cis-regulatory region. The PCPs were isolated by Fluorescent Activated Cell Sorting (FACS) as GFP+ cells at two developmental stages: 8 hpf at ~16C (neurula stage) corresponding to early FGF-mediated induction and 12 hpf at ~18C (tailbud stage) where PCPs are already fate restricted as pigment cells. To avoid a contamination by mesenchyme cells where Ciona enhancers are often ectopically activated, we co-electroporated the MyoD905>YFP construct and counter-selected YFP+ cells. In summary the conditions used are: FGFRDN, Ets:VP16 and Control samples at 8 and 12 hpf (four samples for each condition).

ORGANISM(S): Ciona intestinalis  

SUBMITTER: Claudia Racioppi   Lionel Christiaen   Filomena Ristoratore  

PROVIDER: E-MTAB-2759 | ArrayExpress | 2014-07-14

REPOSITORIES: ArrayExpress

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Publications

Fibroblast growth factor signalling controls nervous system patterning and pigment cell formation in Ciona intestinalis.

Racioppi Claudia C   Kamal Ashwani K AK   Razy-Krajka Florian F   Gambardella Gennaro G   Zanetti Laura L   di Bernardo Diego D   Sanges Remo R   Christiaen Lionel A LA   Ristoratore Filomena F  

Nature communications 20140905


During the development of the central nervous system (CNS), combinations of transcription factors and signalling molecules orchestrate patterning, specification and differentiation of neural cell types. In vertebrates, three types of melanin-containing pigment cells, exert a variety of functional roles including visual perception. Here we analysed the mechanisms underlying pigment cell specification within the CNS of a simple chordate, the ascidian Ciona intestinalis. Ciona tadpole larvae exhibi  ...[more]

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