Dataset Information


Nucleosome plasticity during the cell cycle

ABSTRACT: Nucleosome organization and dynamics play a central role in controlling the DNA accessibility to regulatory factors of many critical cellular functions, especially gene regulation. However, despite extensive studies, the main factors determining nucleosome positioning and its fluctuation during cell cycle still remain elusive. Here, we present a large-scale study of nucleosome plasticity throughout the cell cycle and its interplay with gene expression based on genome-wide nucleosome positioning and mRNA abundance. We have clusterized distinct nucleosome architectures around transcription start sites and replication origins and studied their dynamics during the cell cycle progression. The most significant cell cycle-dependent changes occur at G1-S and G2-M transitions due to a large changes in gene expression in cell cycle regulatory genes. Taken together, our accurate study provides a dynamic picture of chromatin organization along cell cycle and its interplay with gene expression.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Modesto Orozco   Ozgen Deniz   Oscar Flores  

PROVIDER: E-MTAB-2839 | ArrayExpress | 2014-10-09


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Nucleosome architecture throughout the cell cycle.

Deniz Özgen Ö   Flores Oscar O   Aldea Martí M   Soler-López Montserrat M   Orozco Modesto M  

Scientific reports 20160128

Nucleosomes provide additional regulatory mechanisms to transcription and DNA replication by mediating the access of proteins to DNA. During the cell cycle chromatin undergoes several conformational changes, however the functional significance of these changes to cellular processes are largely unexplored. Here, we present the first comprehensive genome-wide study of nucleosome plasticity at single base-pair resolution along the cell cycle in Saccharomyces cerevisiae. We determined nucleosome org  ...[more]

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