Dataset Information


Microarray analysis of in vitro differentiation of adult human pancreatic progenitor cells

ABSTRACT: We have developed an in vitro culture system that allows us to expand progenitor cells from human islet preparations and differentiate them into insulin-producing cells. We noticed however, that cultures from individual islet preparations had very heterogeneous outcomes, from good differentiation to almost none. We therefore speculated that our progenitor cell cultures contained different kinds of cells and that the true endocrine progenitor cells are present in the successful cultures, but not in the unsuccessful ones. To address this issue and to begin to identify markers for the true endocrine progenitor cells we compared global gene expression between a very successful culture (final insulin-expression 10% of islets) and an unsuccessful one (final insulin-expression 0%). We also included RNA from freshly isolated islets for control purposes. The cultures from donor A yielded substantial differentiation, while the cultures from donor B showed no successful differentiation.

INSTRUMENT(S): G2500A Hewlett Packard

ORGANISM(S): Homo sapiens  

SUBMITTER: Joel Habener  

PROVIDER: E-MTAB-3050 | ArrayExpress | 2014-10-31


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