Transcriptomics

Dataset Information

7

Transcriptome study of sleep deprivation-induced ischemic tolerance in rats


ABSTRACT: Sleep deprivation (SD) performed before stroke induces an ischemic tolerance state as observed in other forms of preconditioning. As the mechanisms underlying this effect are not well understood we used DNA oligonucleotide microarray analysis, to identify the genes and the gene-pathways underlying SD preconditioning. Gene expression was analyzed 3 days after stroke surgery in four experimental groups: i) SD performed before focal cerebral ischemia induction; ii) SD performed before Sham surgery; iii) IS without SD; and iv) Sham surgery without SD. SD was performed by gentle handling during the last 6h of the light period and ischemia was induced immediately after. Stroke induced a massive alteration in gene expression both in sleep deprived and non-sleep deprived animals. However, compared to animals that underwent ischemia alone, SD induced a general reduction in transcriptional changes with a reduction in the upregulation of genes involved in cell cycle regulation and immune response. Moreover an upregulation of a new neuroendocrine pathway which included melanin concentrating hormone, glycoprotein hormones-ë±-polypeptide and hypocretin was observed exclusively in rats sleep deprived before stroke. Our data indicate that SD before stroke reprogrammed the signaling response to injury. The inhibition of cell cycle regulation and inflammation are neuroprotective mechanisms reported also for other forms of preconditioning treatment whereas the implication of the neuroendocrine function is novel and has never been described before. These results therefore provide new insights into neuroprotective mechanisms involved in ischemic tolerance mechanisms.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Marta Pace   Francesca Baracchi  

PROVIDER: E-MTAB-3148 | ArrayExpress | 2015-10-30

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-MTAB-3148.idf.txt Idf
E-MTAB-3148.idf.txt_original Idf
E-MTAB-3148.processed.1.zip Processed
E-MTAB-3148.raw.1.zip Raw
E-MTAB-3148.sdrf.txt Txt
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Publications

Identification of Sleep-Modulated Pathways Involved in Neuroprotection from Stroke.

Pace Marta M   Baracchi Francesca F   Gao Bo B   Bassetti Claudio C  

Sleep 20151101 11


<h4>Study objectives</h4>Sleep deprivation (SDp) performed before stroke induces an ischemic tolerance state as observed in other forms of preconditioning. As the mechanisms underlying this effect are not well understood, we used DNA oligonucleotide microarray analysis to identify the genes and the gene-pathways underlying SDp preconditioning effects.<h4>Design</h4>Gene expression was analyzed 3 days after stroke in 4 experimental groups: (i) SDp performed before focal cerebral ischemia (IS) ind  ...[more]

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