Transcriptomics

Dataset Information

590

GENCODE PCR-Seq Batch XIV


ABSTRACT: As part of the ENCODE consortium the GENCODE project is producing a reference gene set through manual and automated gene prediction. Selected transcript models are verified experimentally by RT-PCR amplification of at least one of their unique splice junctions followed by sequencing. The experiment targets are manually annotated transcripts with novel or putative status, non-pseudogene biotype and unique splice junctions not validated previously. For Batch XIV 271 splice junctions from GENCODE16 (released April 2013) were chosen for experimental verification.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens  

SUBMITTER: Electra Tapanari   Roderic Roderic   Jennifer Harrow   Alexandre Reymond  

PROVIDER: E-MTAB-3168 | ArrayExpress | 2014-12-16

SECONDARY ACCESSION(S): ERP009024

REPOSITORIES: ArrayExpress, ENA

altmetric image

Publications


Within the ENCODE Consortium, GENCODE aimed to accurately annotate all protein-coding genes, pseudogenes, and noncoding transcribed loci in the human genome through manual curation and computational methods. Annotated transcript structures were assessed, and less well-supported loci were systematically, experimentally validated. Predicted exon-exon junctions were evaluated by RT-PCR amplification followed by highly multiplexed sequencing readout, a method we called RT-PCR-seq. Seventy-nine perce  ...[more]

Publication: 1/2

Similar Datasets

2014-11-26 | E-MTAB-3122 | ArrayExpress
2014-12-16 | E-MTAB-3169 | ArrayExpress
2017-03-31 | E-MTAB-5527 | ArrayExpress
2013-02-19 | E-MTAB-1505 | ArrayExpress
2012-10-01 | E-MTAB-1310 | ArrayExpress
2016-04-28 | E-MTAB-4644 | ArrayExpress
2015-01-22 | E-MTAB-3261 | ArrayExpress
2016-05-13 | E-MTAB-4644 | ExpressionAtlas
2012-10-01 | E-MTAB-1309 | ArrayExpress
2011-02-26 | E-GEOD-25450 | ArrayExpress