Transcriptomics

Dataset Information

102

Regulation of the ESC transcriptome by nuclear long non-coding RNAs


ABSTRACT: We performed poly(A)+ RNA sequencing (Illumina GAIIx) from nuclear and whole cell RNA fractions of mouse AB2.2 embryonic stem cells (ESCs) and neural progenitors (NPCs) differentiated from ESCs. We further transfected AB2.2 ESCs with control antisense oligonucleotides (ASOs), or 2 independent ASOs each targeting either PAT-14 lncRNA or Firre lncRNA for 24 hours, in 4 biological replicates each and performed poly(A)+ RNA sequencing (Illumina HiSeq 2000).

REANALYSED by: E-MTAB-3198

INSTRUMENT(S): Illumina Genome Analyzer IIx,Illumina HiSeq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: Jan H Bergmann  David L Spector     

PROVIDER: E-MTAB-3198 | ArrayExpress| 2017-01-09

SECONDARY ACCESSION(S): ERP009127

REPOSITORIES: ArrayExpress, ENA

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