Genomics

Dataset Information

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ChIP-seq of Abf1, TATA-binding protein, and RNA Polymerase II in wild type and abf1 temperature sensitive mutant yeast (Saccharomyces cerevisiae)


ABSTRACT: ChIP was performed in wild type and abf1-101 ts yeast after 60 minutes at 37 C

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Saccharomyces cerevisiae S288C  

SUBMITTER: Randall H Morse   

PROVIDER: E-MTAB-3208 | ArrayExpress | 2015-02-01

SECONDARY ACCESSION(S): ERP009156

REPOSITORIES: ArrayExpress, ENA

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Publications

Chromatin mediation of a transcriptional memory effect in yeast.

Paul Emily E   Tirosh Itay I   Lai William W   Buck Michael J MJ   Palumbo Michael J MJ   Morse Randall H RH  

G3 (Bethesda, Md.) 20150305 5


Previous studies have described a transcriptional "memory effect," whereby transcript levels of many Abf1-regulated genes in the budding yeast Saccharomyces cerevisiae are undiminished even after Abf1 has dissociated from its regulatory sites. Here we provide additional support for this effect and investigate its molecular basis. We show that the effect is observed in a distinct abf1 ts mutant from that used in earlier studies, demonstrating that it is robust, and use chromatin immunoprecipitati  ...[more]

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