Dataset Information


Transcriptional characterization of primary human HLA-G+ extravillous trophoblasts (EVT) and Villous trophoblasts (VT)

ABSTRACT: Seven patient paired primary human HLA-G+ extravillous trophoblasts (EVT) and Villous trophoblasts (VT) obtained from 1st trimester (7-9 weeks) villous tissue were obtained. RNA was isolated directly after isolation and purification using FACS sort for CD45-HLA-G+ (EVT) and CD45-HLA-G-EGFR1+ (VT) fractions. Expression profiles were compared to two samples of the choriocarcinoma cell line JEG-3 and four samples of decidual stromal cells (DSC) at passage 2 after cell culture.

ORGANISM(S): Homo sapiens  

SUBMITTER: Jack L Strominger   Tamara Tilburgs  

PROVIDER: E-MTAB-3217 | ArrayExpress | 2015-05-15


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Human HLA-G+ extravillous trophoblasts: Immune-activating cells that interact with decidual leukocytes.

Tilburgs Tamara T   Crespo Ângela C ÂC   van der Zwan Anita A   Rybalov Basya B   Raj Towfique T   Stranger Barbara B   Gardner Lucy L   Moffett Ashley A   Strominger Jack L JL  

Proceedings of the National Academy of Sciences of the United States of America 20150526 23

Invading human leukocyte antigen-G+ (HLA-G+) extravillous trophoblasts (EVT) are rare cells that are believed to play a key role in the prevention of a maternal immune attack on foreign fetal tissues. Here highly purified HLA-G+ EVT and HLA-G- villous trophoblasts (VT) were isolated. Culture on fibronectin that EVT encounter on invading the uterus increased HLA-G, EGF-Receptor-2, and LIF-Receptor expression on EVT, presumably representing a further differentiation state. Microarray and functiona  ...[more]

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