Transcriptomics

Dataset Information

33

Transcriptional profiling of clonal INS-1 832/13 beta-cells


ABSTRACT: This experiment aimed to measure gene expression in glucose-responsive beta-cells. RNA was extracted using RNeasy Mini kit. Quantity and quality of extracted RNA were assessed by the NanoDrop spectrophotometer ND-1000 and Experion RNA StdSens Analysis Kit, respectively. Background correction, normalization, and probe summarization were performed by the Robust Multichip Average method. More details can be found in J Biol Chem (doi: 10.1074/jbc.M112.422527).

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Jiangming Sun  Hindrik Mulder     

PROVIDER: E-MTAB-3232 | ArrayExpress| 2015-01-22

REPOSITORIES: ArrayExpress

altmetric image

Publications

Coordinate changes in histone modifications, mRNA levels, and metabolite profiles in clonal INS-1 832/13 β-cells accompany functional adaptations to lipotoxicity.

Malmgren Siri S   Spégel Peter P   Danielsson Anders P H AP   Nagorny Cecilia L CL   Andersson Lotta E LE   Nitert Marloes Dekker MD   Ridderstråle Martin M   Mulder Hindrik H   Ling Charlotte C  

The Journal of biological chemistry 20130308 17


Lipotoxicity is a presumed pathogenetic process whereby elevated circulating and stored lipids in type 2 diabetes cause pancreatic β-cell failure. To resolve the underlying molecular mechanisms, we exposed clonal INS-1 832/13 β-cells to palmitate for 48 h. We observed elevated basal insulin secretion but impaired glucose-stimulated insulin secretion in palmitate-exposed cells. Glucose utilization was unchanged, palmitate oxidation was increased, and oxygen consumption was impaired. Halting expos  ...[more]

Similar Datasets

2015-08-19 | E-GEOD-65370 | ArrayExpress
2015-10-02 | E-GEOD-23983 | ArrayExpress
2011-06-10 | E-MEXP-2914 | ArrayExpress
2015-05-01 | E-GEOD-17636 | ArrayExpress
2012-02-14 | E-GEOD-35411 | ArrayExpress
2016-03-19 | E-GEOD-79069 | ArrayExpress
2011-06-10 | E-GEOD-12049 | ArrayExpress
2014-07-03 | E-GEOD-17636 | ExpressionAtlas
2013-10-14 | E-GEOD-41332 | ArrayExpress
2014-05-02 | E-MTAB-2 | ArrayExpress