Transcriptomics

Dataset Information

5

Transcription profiling of old and young Laternula elliptica siphon tissue under heat stress


ABSTRACT: Specimens of L. elliptica were collected by scuba divers at a depth of 10-18m in January 2010 at Hangar Cove, Rothera Point, Adelaide Island, Antarctic Peninsula (67°34’07°S, 68°07’30°W). Animals were collected in two size classes: large animals (with lengths ranging around 60mm) and small animals (lengths ranging around 30mm), the sizes of which equated to average ages of 16 and 7 years respectively. These two groups were termed “old” and “young” respectively. The clams were maintained in a flow-through aquarium and allowed to acclimate to laboratory conditions for 2 weeks before experimentation. At the end of the acclimation period, 10 old and 10 young animals were transferred to a 60l jacketed tank with aerated sea water, connected to a thermocirculator. The sea water temperature was gradually raised from 0°C to +3°C over a 12 hour period. This temperature was then maintained for a further 12 hours, before sampling the animals. Siphon tissue samples were dissected and immediately snap frozen in liquid nitrogen and stored at -80°C. The sampling regime was repeated on 10 old and 10 young animals that had been maintained in the flow-through aquarium for the same time period (control animals) RNA was extracted from all samples using TriSure, according to manufacturer’s instructions. RNAs from siphon tissue from 5 animals from each group (old treated, young treated, old control and young control) were used in the array hybridization experiments. The array used was A-MEXP-1676. PCR amplified labelled cDNA targets were prepared from 1μg total RNA. 5 replicates were used.

ORGANISM(S): Laternula elliptica  

SUBMITTER: Michael Thorne  

PROVIDER: E-MTAB-3284 | ArrayExpress | 2016-02-29

REPOSITORIES: ArrayExpress

Dataset's files

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Action DRS
E-MTAB-3284.idf.txt Idf
E-MTAB-3284.idf.txt_original Idf
E-MTAB-3284.raw.1.zip Raw
E-MTAB-3284.sdrf.txt Txt
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Publications

Age-related thermal response: the cellular resilience of juveniles.

Clark M S MS   Thorne M A S MAS   Burns G G   Peck L S LS  

Cell stress & chaperones 20150912 1


Understanding species' responses to environmental challenges is key to predicting future biodiversity. However, there is currently little data on how developmental stages affect responses and also whether universal gene biomarkers to environmental stress can be identified both within and between species. Using the Antarctic clam, Laternula elliptica, as a model species, we examined both the tissue-specific and age-related (juvenile versus mature adult) gene expression response to acute non-letha  ...[more]

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