Dataset Information


Transcription profiling by array of zebrafish larvae exposed to relative microgravity

ABSTRACT: The general objective of the study was to determine modulation of gene expression by environmental factors, with a special emphasis on bone formation. For this reason, the specific period of treatment was chosen between 5-6 days post-fertilization (dpf), when bone formation and calcification are taking place. This experiment was designed as a new type of gravitational experiment, which we like to call \relative microgravity\, referring to the fact that the larvae first grow in hyper gravity for 5 days and are then returned to 1g normal gravity for 1 day. Zebrafish embryos were placed on a Large Diameter Centrifuge at 3 hpf, brought to a gravitational force of 3 g until 5 dpf. Reference embryos were kept in parallel at 1g (Inc). At 5dpf, one batch was left at 3g (3g), one batch was returned to 1g (3g>1g), while a third batch was returned to 1g, but left on the axis of the centrifuge (Axe; 3g>Axe). The experiment was repeated 4 times, each time with 4 batches of 60 larvae.


ORGANISM(S): Danio rerio  

SUBMITTER: Rasoul Nourizadeh   Marc Muller  

PROVIDER: E-MTAB-3290 | ArrayExpress | 2015-02-27


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Zebrafish Bone and General Physiology Are Differently Affected by Hormones or Changes in Gravity.

Aceto Jessica J   Nourizadeh-Lillabadi Rasoul R   Marée Raphael R   Dardenne Nadia N   Jeanray Nathalie N   Wehenkel Louis L   Aleström Peter P   van Loon Jack J W A JJ   Muller Marc M  

PloS one 20150610 6

Teleost fish such as zebrafish (Danio rerio) are increasingly used for physiological, genetic and developmental studies. Our understanding of the physiological consequences of altered gravity in an entire organism is still incomplete. We used altered gravity and drug treatment experiments to evaluate their effects specifically on bone formation and more generally on whole genome gene expression. By combining morphometric tools with an objective scoring system for the state of development for eac  ...[more]

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