Transcriptomics

Dataset Information

65

Development and validation of a mixed-tissue oligo microarray for Atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758)


ABSTRACT: The largest of the tuna species, Atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758), inhabits the North Atlantic Ocean and the Mediterranean Sea and is considered to be an endangered species, largely through overfishing. Thus, the development of aquaculture practices independent of wild resources can provide an important contribution towards ensuring security and sustainability of this species in the longer-term. In order to provide a resource for ongoing studies, we have used 454 pyrosequencing technology to sequence a mixed-tissue normalized cDNA library, derived from adult individuals. Transcript sequences were used to develop a novel 15K Agilent oligo microarray for T. thynnus and comparative tissue gene expression profiles were inferred for gill, heart, liver, ovaries and testes.

ORGANISM(S): Thunnus thynnus  

SUBMITTER: Željka Trumbić  

PROVIDER: E-MTAB-3412 | ArrayExpress | 2015-08-10

REPOSITORIES: ArrayExpress

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Publications

Development and validation of a mixed-tissue oligonucleotide DNA microarray for Atlantic bluefin tuna, Thunnus thynnus (Linnaeus, 1758).

Trumbić Željka Ž   Bekaert Michaël M   Taggart John B JB   Bron James E JE   Gharbi Karim K   Mladineo Ivona I  

BMC genomics 20151125


The largest of the tuna species, Atlantic bluefin tuna (Thunnus thynnus), inhabits the North Atlantic Ocean and the Mediterranean Sea and is considered to be an endangered species, largely a consequence of overfishing. T. thynnus aquaculture, referred to as fattening or farming, is a capture based activity dependent on yearly renewal from the wild. Thus, the development of aquaculture practices independent of wild resources can provide an important contribution towards ensuring security and sust  ...[more]

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