Transcriptomics

Dataset Information

17

Analysis of gene expression changes in response to hypoxia and/or cisplatin in A2780 and A2780cis cells


ABSTRACT: The aim of this experiment was to evaluate the changes in gene expression in response to hypoxia and/or cisplatin in an ovarian cancer cell line model. A2780 (cisplatin-sensitive) and A2780cis (cisplatin-resistant) cell lines were treated with cisplatin in normoxia or hypoxia (0.5% O2) for 72 hours. RNA was extracted from three independent biological replicates for each condition: A2780 (normoxia, untreated); A2780 (hypoxia, untreated); A2780 (normoxia, cisplatin); A2780cis (hypoxia, cisplatin), A2780cis (normoxia, untreated); A2780cis (hypoxia, untreated); A2780cis (normoxia, cisplatin); A2780cis (hypoxia, cisplatin) and interrogated on Affymetrix Human Gene ST 1.0 arrays.

REANALYSED by: E-MTAB-3645

INSTRUMENT(S): Genechip scanner, INVIVO2 hypoxia chamber

ORGANISM(S): Homo sapiens  

SUBMITTER: Lynda McEvoy  

PROVIDER: E-MTAB-3645 | ArrayExpress | 2016-01-15

REPOSITORIES: ArrayExpress

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Publications

Identifying novel hypoxia-associated markers of chemoresistance in ovarian cancer.

McEvoy Lynda M LM   O'Toole Sharon A SA   Spillane Cathy D CD   Martin Cara M CM   Gallagher Michael F MF   Stordal Britta B   Blackshields Gordon G   Sheils Orla O   O'Leary John J JJ  

BMC cancer 20150725


Ovarian cancer is associated with poor long-term survival due to late diagnosis and development of chemoresistance. Tumour hypoxia is associated with many features of tumour aggressiveness including increased cellular proliferation, inhibition of apoptosis, increased invasion and metastasis, and chemoresistance, mostly mediated through hypoxia-inducible factor (HIF)-1α. While HIF-1α has been associated with platinum resistance in a variety of cancers, including ovarian, relatively little is know  ...[more]

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