Transcriptomics

Dataset Information

300

Genome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding


ABSTRACT: We investigated the effect that intragenic s54 binding sites have on transcripton elongation. In an rpoN deletion we have over-expressed rpoN from a plasmid (with vector only contol) and then looked at changes in transcription elongation around intragenic s54 binding sites by RNA-seq. We are also looking at changes in transcription initiation/elongation at sites of sigma factor overlap.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Escherichia coli  

SUBMITTER: Richard P Bonocora   Pascal Lapierre   Joseph T Wade   Carol Smith  

PROVIDER: E-MTAB-3699 | ArrayExpress | 2015-08-06

SECONDARY ACCESSION(S): ERP010917

REPOSITORIES: ArrayExpress, ENA

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