Dataset Information


Transcription profiling of brown adipose tissue from resistin-transgenic spontaneously hypertensive rats

ABSTRACT: Resistin has been originally identified as an adipokine that links obesity to insulin resistance in mice. In our previous studies in spontaneously hypertensive rats (SHR) expressing a nonsecreted form of mouse resistin (Retn) transgene specifically in adipose tissue (SHR-Retn), we observed an increased lipolysis and serum free fatty acids, ectopic fat accumulation in muscles and insulin resistance. Recently, brown adipose tissue (BAT) has been suggested to play an important role in the pathogenesis of metabolic disturbances by its ability to dissipate energy excess. In the current study, we analyzed autocrine effects of transgenic resistin on BAT glucose and lipid metabolism and mitochondrial function in the SHR-Retn versus nontransgenic SHR controls. We observed that interscapular BAT isolated from SHR-Retn transgenic rats when compared to SHR controls showed a lower relative weight, significantly reduced both basal and insulin stimulated incorporation of palmitate into BAT lipids, and significantly decreased palmitate oxidation and glucose oxidation. In addition, in vivo microPET imaging revealed significantly reduced 18F-FDG uptake in BAT induced by exposure to cold in SHR-Retn versus control SHR. Gene expression profiles identified differentially expressed genes involved in skeletal muscle and connective tissue developmental and inflammation, as well as MAPK and insulin signaling. These results provide compelling evidence that autocrine effects of resisitin in BAT play an important role in the pathogenesis of insulin resistance in the rat.


ORGANISM(S): Rattus norvegicus  

SUBMITTER: Hynek Strnad   Michal Pravenec   Tomas Mracek  Vaclav Zidek  Ludmila Kazdova  Katerina E Henke  Hana Malinska  Josef Houstek  Petr Mlejnek  Zdenek Drahota  Vladimir Landa  Vojtech Skop  Sebastian Eigner  Jan Silhavy  Miroslava Simakova 

PROVIDER: E-MTAB-3787 | ArrayExpress | 2016-07-03


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