Gene expression profile of normal thyroid tissue as indicator of thyroid carcinoma during thyroidectomy
ABSTRACT: In the present study, we examine expression profiles of normal tissue fragments, intraoperatively taken on the basis of macroscopic judgment and confirmed by immunohistochemistry, from patients with neoplastic and non-neoplastic disease of thyroid. The distinct GEP detected may influence the insurgence and/or progression of thyroid cancer, its molecular classification, and thus pinpoint selective gene targets for new preoperative diagnostic approaches treatments and prophylactic strategies.
Gene expression profiling (GEP) of normal thyroid tissue from 43 patients with thyroid carcinoma, 6 with thyroid adenoma, 42 with multinodular goiter, and 6 with Graves-Basedow disease was carried out with the aim of achieving a better understanding of the genetic mechanisms underlying the role of normal cells surrounding the tumor in the thyroid cancer progression. Unsupervised and supervised analyses were performed to compare samples from neoplastic and non-neoplastic diseases. GEP and subsequ ...[more]
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