Dataset Information


Cyclin D1 ChIP-seq in H9 undifferentiated human pluripotent stem cells

ABSTRACT: H9 human pluripotent stem cells were grown in chemically defined media with Activin A and FGF2. Four 10cm plates with 70% confluency were used per sample. Cells were cross-linked by adding a final concentration of 1% PFA into culture media and incubated for 10 min at RT for Cyclin D1 ChIP. ChIP-seq experiment was performed in duplicate.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens  

SUBMITTER: Pedro Madrigal   Ludovic Vallier   Siim Pauklin   Alessandro Bertero  

PROVIDER: E-MTAB-3807 | ArrayExpress | 2016-02-01



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Initiation of stem cell differentiation involves cell cycle-dependent regulation of developmental genes by Cyclin D.

Pauklin Siim S   Madrigal Pedro P   Bertero Alessandro A   Vallier Ludovic L  

Genes & development 20160201 4

Coordination of differentiation and cell cycle progression represents an essential process for embryonic development and adult tissue homeostasis. These mechanisms ultimately determine the quantities of specific cell types that are generated. Despite their importance, the precise molecular interplays between cell cycle machinery and master regulators of cell fate choice remain to be fully uncovered. Here, we demonstrate that cell cycle regulators Cyclin D1-3 control cell fate decisions in human  ...[more]

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