Transcriptomics,Genomics

Dataset Information

97

Otx2 binding to Nanog promoter protects embryonal stem cells from corruption of naive pluripotency and is required at morula stage for inner-cell mass differentiation of the epiblast


ABSTRACT: We have investigated the functional relevance of the transcriptional network controlled by the Otx2 binding activity in ESCs, EpiSCs and in vivo. In particular, to study the Otx2 transcriptional network we first performed Otx2 chromatin immunoprecipitation sequencing (ChIP-seq) experiments in wt ESCs and day 4 (d4) ESC-derived EpiSCs and employed as negative control ESCs and EpiSCs lacking Otx2. Moreover, to investigate the functional relevance of the Otx2 binding activity in pluripotent stem cells, we mutagenized in ESCs the Otx2 binding site located upstream of the Nanog TSS. To characterize the identity of ESCs and EpiSCs both lacking this Otx2 binding site, we analyzed their transcriptome by RNA-Seq.

INSTRUMENT(S): Illumina HiSeq 1000,Illumina HiSeq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: Marco Savarese  Antonio Simeone  Dario Acampora       

PROVIDER: E-MTAB-3856 | ArrayExpress| 2016-05-31

SECONDARY ACCESSION(S): ERP011969

REPOSITORIES: ArrayExpress, ENA

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-01-23 | E-GEOD-54084 | ArrayExpress
2015-08-29 | E-GEOD-66670 | ArrayExpress
2012-07-17 | E-GEOD-22875 | ArrayExpress
| GSE22875 | GEO
| GSE81494 | GEO
2015-08-20 | E-GEOD-69724 | ArrayExpress
2014-05-30 | E-GEOD-52006 | ArrayExpress
2015-08-29 | E-GEOD-66671 | ArrayExpress
2011-05-31 | E-GEOD-21900 | ArrayExpress
| GSE69547 | GEO