Transcriptomics

Dataset Information

32

Identification of genes enriched in nociceptive neurons of Drosophila larvae


ABSTRACT: This study aims to identify genes whose expression is enriched in the nociceptive neurons of Drosophila larvae. These neurons are involved in detecting noxious mechanical and noxious thermal stimuli. Four replicates each for Class I md-da neurons (2-21Gal4>>UAS-mCD8GFP) and Class IV md-da (ppk-GAL4>>UAS-mCD8GFP) neurons were analyzed. RNA from 50-60 neurons for each replicate was isolated by laser capture microdissection. RNA was amplified using the WT-Ovation Pico Kit from NuGen technologies. RNA was fragmented and labeled using the FL-Ovation cDNA Biotin Module V2 (NuGen Technologies). RNA was hybridized to arrays and data scanned at the Translational Genomics Research Institute.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: W. Daniel Tracey   

PROVIDER: E-MTAB-3863 | ArrayExpress| 2016-01-02

REPOSITORIES: ArrayExpress

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