Transcription profiling by array of pig intestinal cells exposed to Zearalenone or E. coli contamination
ABSTRACT: This is a cell culture based study to asses the impact of ZEA (Zearalenone) and E. coli co-contamination on IPEC cells, these is a normal epithelial cell line isolated from a new born piglet. ZEA is a mycotoxin with a negative impact in human health. The microarray is a custom Agilent Technology array slide with the AMAID: 05685.
The toxicity of zearalenone (ZEA) was evaluated in swine spleen, a key organ for the innate and adaptative immune response. Weaned pigs were fed for 18 days with a control or a ZEA contaminated diet. The effect of ZEA was assessed on wide genome expression, pro- (TNF-α, IL-8, IL-6, IL-1β, IFN-γ) and anti-inflammatory (IL-10, IL-4) cytokines, other molecules involved in inflammatory processes (MMPs/TIMPs), as well as signaling molecules, (p38/JNK1/JNK2-MAPKs) and nuclear receptors (PPARγ/NFkB/AP- ...[more]
Project description:Comparing the expression profiles of the genes in response to estrogen or 17b-estradiol (E2) and a mycotoxin, zearalenone (ZEA), and its analogues. Keywords: Gene expression profiling Overall design: Two-condition experiment, E2- or ZEA-treated vs. control cells. 2 to 3 biological replicates, independently grown and harvested. Two to three replicates per array.
Project description:Comparing the expression profiles of the genes in response to estrogen or 17b-estradiol (E2) and a mycotoxin, zearalenone (ZEA), and its analogues. Keywords: Gene expression profiling Two-condition experiment, E2- or ZEA-treated vs. control cells. 2 to 3 biological replicates, independently grown and harvested. Two to three replicates per array.
Project description:Proteomic changes associated with the individual and combined exposures of deoxynivalenol (DON) and zearalenone (ZEA) were investigated in human hepatocytes (HepaRG cell line) after 24 hour expousre and at low cytotoxicity levels using liquid chromatography coupled to tandem mass spectrometry.
Project description:Proteomic changes associated with the individual and combined exposures of deoxynivalenol (DON) and zearalenone (ZEA) were investigated in human hepatocytes (HepaRG cell line) after only 1h exposure and at low cytotoxicity levels using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)
Project description:The differentiation of specialized feeding sites in Zea mays root cells in response to nematode infestation involves substantial cellular reprogramming of host cells that is not well characterized at the molecular level. Expression data was generated from Zea mays root cells undergoing giant cell formation due to nematode infestation and from non-infested control root cells. Cells were laser captured 14 and 21 days after infestation. Each time point (14 day and 21 day) consisted of three biological replicates per treatment (control root cells or giant cells). Control cells were captured from an area ~13,000,000 um2 in size and giant cells were captured from an area ~5,000,000 um2 in size. RNA samples were isolated using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, USA). RNA amplifications were carried out with the NuGEN WT-Ovation Pico kit.
Project description:Anthracnose caused by the ascomycete Colletotrichum graminicola is one of the most severe fungal diseases of Zea maize. Cultivars with different levels of resistance have been described. However, which genes contribute to cultivar-specific constitutive and/or induced defense in this economically important pathosystem is still elusive. Transcriptome analyses of infected maize leaves of varieties Golden Jubilee (GJ) and B73 by RNA-Seq was performed for the penetration, biotrophic and necrotrophic phases.
Project description:The Helicoverpa armigera single-capsid nucleopolyhedrovirus (HaSNPV) can be propagated using H. zea insect cell cultures, for use as a biopesticide against Heliothine agricultural pests.This study sequenced, assembled and functionally annotated 29,586 transcript sequences from cultured H. zea cells using Illumina 100 bps and paired-end transcriptome sequencing (RNA-seq). From these sequences, a genome-scale microarray platform was constructed and validated for effective expression analysis of H. zea genes. This array also included probes for all HaSNPV genes, thereby allowing virus and host gene changes to be monitored simultaneously. A 4x180,000 SurePrint Agilent expression array (Agilent, Santa Clara, CA) was employed so that a high number of probes can be included to test all 29,586 assembled H. zea sequences and to eventually select a best probe for each transcript. Two biological replicates for uninfected H. zea cells and two other biological replicates for infected samples at 18 hours post infection were analyzed. Six Agilent 60-mer oligonucleotide probes were designed by eArray (Agilent) for each transcript, in which each orientation had three probes randomly distributed across the sequences. Probes that had potential cross-hybridization (Xhyb) were removed. The final probe set for H. zea sequences included 153,583 probes. In addition, probes for all 135 H.armigera single-capsid nucleopolyhedrovirus (HaSNPV) genes were added to investigate host-virus interaction in culture.