Name: Transcriptome profiling of Sinorhizobium meliloti Rm1021 grown in semisolid media (MM) in presence of 2-tridecanone (2-TDC)
Keywords: transcription profiling by array

OmicsDI

Browse
Submit Data
Databases
API
Help
Login

Dataset Information

119 Views

1 Connections

0 Citations

0 Reanalyses

4 Downloads

Omics score: 37

Transcriptome profiling of Sinorhizobium meliloti Rm1021 grown in semisolid media (MM) in presence of 2-tridecanone (2-TDC)

ABSTRACT: This experiment captures the genes differentially expressed of the Sinorhizobium meliloti reference strain (Rm1021) when this bacteria is grown in semisolid media (MM 0.6%) in presence of a compound which promotes surface motiliy

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: LYDIA BERNABÉU

PROVIDER: E-MTAB-3893 | ArrayExpress | 2018-06-29

REPOSITORIES: ArrayExpress

ACCESS DATA

Json Xml

Dataset's files

Source:

primary

			Action	DRS
	E-MTAB-3893.idf.txt	Idf
	E-MTAB-3893.idf.txt_original	Idf
	E-MTAB-3893.raw.1.zip	Raw
	E-MTAB-3893.sdrf.txt	Txt

Items per page:

1 - 4 of 4

Similar Datasets

Transcription profiling of Sinorhizobium meliloti wild type and Hfq ORF deletion strains

Project description:Hfq-dependent transcriptional alterations in the nitrogen-fixing endosymbiont S. meliloti. Comparison: S. meliloti 1021 strain Vs S. meliloti 1021Dhfq (containing a deletion of the hfq ORF).

2010-03-11 | E-MEXP-2501 | ArrayExpress

Transcription profiling by array of Sinorhizobium meliloti 1021 and 1021rhbD grown on semisolid minimal medium

Project description:Growth of Sinorhizobium meliloti 1021 and 1021rhbD on semisolid minimal medium during 14 hours

2013-03-01 | E-MEXP-3769 | ArrayExpress

Transcription profiling by array of Sinorhizobium meliloti 1021RctR compared with wild-type 1021 in order to identify genes differentially expressed in a 1021RctR mutant

Project description:RctR encodes a protein belonging to the GntR family of transcriptional regulators and is involved in regulation of conjugative transfer of symbiotic plasmids of Sinorhizobium meliloti. In order to identify genes differentially expressed in a 1021RctR mutant, the transcriptome of S. meliloti 1021RctR was compared with that of the wild-type 1021, using Sm6kOligo DNA microarrays. Cells of 1021 and 1021RctR were grown at 30C in TY broth to mid logarothmic phase (OD600nm)=0.7-0.8 before RNA isolation.

2012-09-03 | E-MEXP-3664 | ArrayExpress

Transcription profiling time series of Sinorhizobium meliloti in response to an osmotic upshift elicited by salt or sucrose

Project description:Time course of the S. meliloti response to an osmotic upshift elicited by salt (300mM or 400mM) or sucrose (500mM or 700mM)

2006-08-17 | E-MEXP-785 | ArrayExpress

Transcription profiling by array of Sinorhizobium meliloti 1021 and 1021FDC5 strains

Project description:Transcriptome profiling of S. meliloti 1021 and 1021FDC5 strains growth in minimal media since A600 1.1.

2010-06-09 | E-MEXP-2651 | ArrayExpress

Transcription profiling of Sinorhizobium meliloti strain 1021FDC5 grown in liquid, solid or semi-solid media

Project description:S. meliloti 1021FDC5 was grown at 30ºC in 20 ml of TY broth with shaking to late logarithmic phase (optical density at 600 nm = 1-1.2). After incubation, cells were pelleted, washed twice in MM and resuspended in 2 ml of the latter medium. For time course experiments in liquid, 0.5 ml of the inoculation culture was added to 50 ml of fresh MM. At various times, samples were removed for determining viable cells counts as well as for RNA isolation/preparation (7 and 14 hours). For experiments on plates, 20 ml of MM containing 0.7% (Semisolid) or 1.3% (Solid) agar was dispensed onto each Petri dish and allowed to gel. The plates were air dried at room temperature for 15 min. 0.1 ml of the inoculation culture was plated onto the surface of the plates and allowed to dry for 10 min. The plates were then inverted and incubated at 30ºC.

2009-04-03 | E-MEXP-1953 | ArrayExpress

GNS577_transcriptome

Project description:Transcriptome profiling by array of the Sinorhizobium meliloti GR4-derivative mutant GNS577, affected in ntrY and ntrX genes, to identify possible cell processes regulated by this system

2017-04-30 | E-MTAB-2511 | ArrayExpress

Comparison of the transcriptional profiles of Sinorhizobium meliloti wildtype 1021 and two S. meliloti mutants deficient in phospholipid biosynthesis

Project description:S. meliloti genes differently expressed in the wildtype 1021, the phosphatidylethanolamine-deficient mutant CS111, or the phosphatidylcholine-deficient mutant OG10017 were identified by determining and comparing the whole genome transcription profiles of these three strains. Mutants and wildtype present different phenotypes and the molecular bases for these differences was explored by transcriptome analysis.

2021-06-29 | E-MTAB-3676 | ArrayExpress

Mouse infections with Neospora caninum

Project description:Transcriptional responses occurring in the spleen in response to N. caninum infection compared to uninfected mice Two conditions: infected and un-infected mice; Biological replicates: three (or four) infected and three uninfected mice. One control and one infected per array

2011-01-01 | E-GEOD-23520 | ArrayExpress