Dataset Information


Transcriptional profiling of acinar cells from mice engineered with 3 TFs for reprogramming to beta cells

ABSTRACT: This experiment used RNA-Seq technology to explore gene expression in mouse FACS-purified pancreatic P60 Acinar cells, P60 Beta-cells, 1 day 3TF (MafA/Neurog3/Pdx1)-induced Acinar cells and 7 days 3TF-induced Acinar cells.ďż˝

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: Mark A Magnuson   Hannah W Clayton   Elisabetta Manduchi   Christian J Stoeckert  

PROVIDER: E-MTAB-3921 | ArrayExpress | 2016-07-06



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Using a transgenic mouse model to express MafA, Pdx1, and Neurog3 (3TF) in a pancreatic acinar cell- and doxycycline-dependent manner, we discovered that the outcome of transcription factor-mediated acinar to β-like cellular reprogramming is dependent on both the magnitude of 3TF expression and on reprogramming-induced inflammation. Overly robust 3TF expression causes acinar cell necrosis, resulting in marked inflammation and acinar-to-ductal metaplasia. Generation of new β-like cells requires l  ...[more]

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