Transcriptomics

Dataset Information

1

Transcription profiling by array of Arabidopsis thaliana Col‑0 wild-type, Col-0 nrpe1-11, and Col-0 ros1-4 at 48 and 72 hours after inoculation with Hyaloperonospora arabidopsidis strain WACO9


ABSTRACT: The experiment was designed to gain more insight into the role of DNA (de)methylation in the regulation of Arabidopsis thaliana gene transcription during a compatible interaction with the downy mildew pathogen Hyaloperonospora arabidopsidis strain WACO9 (Hpa). For this study, comparisons were made between Col-0 (wild-type), nrpe1-11 (which is affected in DNA methylation and globally hypo-methylated) and ros1-4 (impaired in DNA de-methylation and globally hyper-methylated). The latter two mutants have opposite phenotypes with respect to basal resistance and salicylic acid-dependent defence against Hpa. Samples were taken at 48 and 72 hours after spray-inoculation with either 10^5 spores ml^-1 Hyaloperonospora arabidopsidis strain WACO9 in water or water only (mock).

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Jurriaan Ton   Ana López-Sánchez   Joost HM Stassen  

PROVIDER: E-MTAB-3963 | ArrayExpress | 2016-08-03

REPOSITORIES: ArrayExpress

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Publications

The role of DNA (de)methylation in immune responsiveness of Arabidopsis.

López Sánchez Ana A   Stassen Joost H M JH   Furci Leonardo L   Smith Lisa M LM   Ton Jurriaan J  

The Plant journal : for cell and molecular biology 20160907 3


DNA methylation is antagonistically controlled by DNA methyltransferases and DNA demethylases. The level of DNA methylation controls plant gene expression on a global level. We have examined impacts of global changes in DNA methylation on the Arabidopsis immune system. A range of hypo-methylated mutants displayed enhanced resistance to the biotrophic pathogen Hyaloperonospora arabidopsidis (Hpa), whereas two hyper-methylated mutants were more susceptible to this pathogen. Subsequent characteriza  ...[more]

Publication: 1/2

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