Transcriptomics

Dataset Information

297

Continuous CRISPR spacer acquisition in STSV2-infected crenarchaeon Sulfolobus islandicus REY15A over a 6-day period


ABSTRACT: A whole transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich (TYS) and basal (SCV) media over a 6 day period. Spacer acquisition preceded strong host growth retardation, and changes in viral transcript abundance and virus copy numbers showed significant differences between the two media. Results showed that rich medium favoured CRISPR-Cas immunity generation.

INSTRUMENT(S): TRIzol (Sigma Aldrich), Illumina MiSeq, ScriptSeq complete kit Low input (Bacteria) (Epicentre), Illumina HiSeq 2000

ORGANISM(S): Sulfolobus islandicus  

SUBMITTER: Carlos Len-Sobrino   Witold P Kot   Roger A Garrett  

PROVIDER: E-MTAB-4027 | ArrayExpress | 2015-12-06

SECONDARY ACCESSION(S): ERP013310

REPOSITORIES: ArrayExpress, ENA

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Publications

Transcriptome changes in STSV2-infected Sulfolobus islandicus REY15A undergoing continuous CRISPR spacer acquisition.

León-Sobrino Carlos C   Kot Witold P WP   Garrett Roger A RA  

Molecular microbiology 20151127 4


A transcriptome study was performed on Sulfolobus islandicus REY15A actively undergoing CRISPR spacer acquisition from the crenarchaeal monocaudavirus STSV2 in rich and basal media over a 6 day period. Spacer acquisition preceded strong host growth retardation, altered transcriptional activity of four different CRISPR-Cas modules and changes in viral copy numbers, and with significant differences in the two media. Transcript levels of proteins involved in the cell cycle were reduced, whereas tho  ...[more]

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