Transcriptomics,Multiomics

Dataset Information

6

Transcription profiling by high throughput sequencing of seed, spikelet and stem tissues of Sorghum bicolor Btx623


ABSTRACT: This study used with RNA-Seq to examine the tissue specific expression data within sorghum plants for improving the Sorghum bicolor gene annotation. We examined the RNA from tissues (spikelet, seed and stem) in Sorghum bicolor (BTx623).Total RNAs form each tissues were extracted using SDS/phenol method followed by LiCl purification

REANALYSED by: E-MTAB-4273

OTHER RELATED OMICS DATASETS IN: PRJDB3281PRJDB3280

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Sorghum Bicolor

TISSUE(S): Seed, Spikelet, Stem

SUBMITTER: Minami Matsui  

PROVIDER: E-MTAB-4273 | ArrayExpress | 2015-03-11

REPOSITORIES: ArrayExpress

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Publications

MOROKOSHI: transcriptome database in Sorghum bicolor.

Makita Yuko Y   Shimada Setsuko S   Kawashima Mika M   Kondou-Kuriyama Tomoko T   Toyoda Tetsuro T   Matsui Minami M  

Plant & cell physiology 20141209 1


In transcriptome analysis, accurate annotation of each transcriptional unit and its expression profile is essential. A full-length cDNA (FL-cDNA) collection facilitates the refinement of transcriptional annotation, and accurate transcription start sites help to unravel transcriptional regulation. We constructed a normalized FL-cDNA library from eight growth stages of aerial tissues in Sorghum bicolor and isolated 37,607 clones. These clones were Sanger sequenced from the 5' and/or 3' ends and in  ...[more]

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