Transcriptomics

Dataset Information

598

Transcription profiling by high throughput sequencing of the hexaploid wheat line N9134 inoculated with Stripe rust and powdery mildew


ABSTRACT: Stripe rust (Puccinia striiformis f. sp. tritici; Pst) and powdery mildew (Blumeria graminis f. sp. tritici; Bgt) are important diseases of wheat (Triticum aestivum) worldwide. Similar mechanisms and gene transcripts are assumed to be involved in the host defense response because both pathogens are biotrophic fungi. The main objective of our study was to identify co-regulated mRNAs that show a change in expression pattern after inoculation with Pst or Bgt, and to identify mRNAs specific to the fungal stress response. In the present study, cDNA libraries were constructed from leaves inoculated with Pst or Bgt at 0, 1, 2 and 3 days post-inoculation (dpi) with three biological replicates, and then sequenced using the Illumina HiSeq™ 2000 platform. Note: Samples in SRA were assigned the same sample accession. This is incorrect as there are different samples, hence “Source Name” was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.

REANALYSED by: E-MTAB-4289

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Triticum aestivum  

SUBMITTER: Hong Zhang  

PROVIDER: E-MTAB-4289 | ArrayExpress | 2015-02-13

SECONDARY ACCESSION(S): SRP041017

REPOSITORIES: ArrayExpress, ENA

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Publications

Large-scale transcriptome comparison reveals distinct gene activations in wheat responding to stripe rust and powdery mildew.

Zhang Hong H   Yang Yongzheng Y   Wang Changyou C   Liu Min M   Li Hao H   Fu Ying Y   Wang Yajuan Y   Nie Yingbin Y   Liu Xinlun X   Ji Wanquan W  

BMC genomics 20141015


BACKGROUND: Stripe rust (Puccinia striiformis f. sp. tritici; Pst) and powdery mildew (Blumeria graminis f. sp. tritici; Bgt) are important diseases of wheat (Triticum aestivum) worldwide. Similar mechanisms and gene transcripts are assumed to be involved in the host defense response because both pathogens are biotrophic fungi. The main objective of our study was to identify co-regulated mRNAs that show a change in expression pattern after inoculation with Pst or Bgt, and to identify mRNAs speci  ...[more]

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