Transcriptomics

Dataset Information

298

Transcription profiling by high throughput sequencing of rice grain from from heat-tolerant and -sensitive lines in response to high night temperatures at the early milky stage


ABSTRACT: We analyzed the transcriptome profiles for rice grain from heat-tolerant and -sensitive lines in response to high night temperatures at the early milky stage using the Illumina Sequencing method. On the 8th day after the labeled florets flowered, plants with the same label were transferred to chambers and maintained at a temperature of 38.0 ± 0.5°C (treatment) or 25.0 ± 0.5°C (control) for the dark period (10 h), and 26.0 ± 0.5°C (both treatment and control) for the light period (14 h). Three biological replicates of the temperature treatments were grown under the same conditions. After 48 h of treatment, samples containing 45 grains with labels from the same region (middle to bottom part) of labelled ears were harvested, packed in aluminum foil, and flash-frozen in liquid nitrogen until further use. A total of 12 rice grain samples were harvested, i.e., controls (TC1, TC2 and TC3) and treatments (TT1, TT2 and TT3) of the three biological replicates of the heat-tolerant line, and controls (SC1, SC2 and SC3) and treatments (ST1, ST2 and ST3) of the three biological replicates of the heat-sensitive line.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): metagenome  

SUBMITTER: Jiang-Lin Liao  

PROVIDER: E-MTAB-4348 | ArrayExpress | 2014-12-01

SECONDARY ACCESSION(S): SRP050315

REPOSITORIES: ArrayExpress, ENA

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Publications

Transcriptome changes in rice (Oryza sativa L.) in response to high night temperature stress at the early milky stage.

Liao Jiang-Lin JL   Zhou Hui-Wen HW   Peng Qi Q   Zhong Ping-An PA   Zhang Hong-Yu HY   He Chao C   Huang Ying-Jin YJ  

BMC genomics 20150123


<h4>Background</h4>Rice yield and quality are adversely affected by high temperatures, especially at night; high nighttime temperatures are more harmful to grain weight than high daytime temperatures. Unfortunately, global temperatures are consistently increasing at an alarming rate and the minimum nighttime temperature has increased three times as much as the corresponding maximum daytime temperature over the past few decades.<h4>Results</h4>We analyzed the transcriptome profiles for rice grain  ...[more]

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