Transcriptomics

Dataset Information

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Transcription profiling by high throughput sequencing of mouse Ptf1a^YFP/+ early pancreatic multipotent progenitor cells, nascent acinar cells and Ptf1a^YFP/YFP null delayed early multipotent progenitor cells


ABSTRACT: This experiment used RNA-Seq technology to explore gene expression in mouse Ptf1a^YFP/+ [het] FACS sorted cells at E11.5 (early pancreatic Multipotent Progenitor Cells) and E15.5 (nascent acinar cells) as well as in Ptf1a^YFP/YFP [null] at E11.5 (delayed early MPC). 376 selected genes identified as differentially expressed between early pancreatic MPC and nascent acinar cells or between early pancreatic and delayed early MPCs have then been examined by Taqman Low Density Arrays (TLDAs) with Real Time RT-PCR for each 1-day time point from E10.5 to E15. 5 in Ptf1a^YFP/+ [het] and for E10.5 and E11.5 in Ptf1aYFP/YFP [null] . Finally, 94 genes identified in the first phase of TLDAs (including 2 endogenous control, Gapdh and 18S) were analyzed in a second TLDA phase for each 1-day time point from E10.5 to -E18.5 in Ptf1a^YFP/+ [het] and for E11.5 in Ptf1aYFP/YFP [null] with biological replicates (n>=3) for each time point.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Mus musculus  

SUBMITTER: Mark Magnuson   Leah Potter  

PROVIDER: E-MTAB-449 | ArrayExpress | 2014-11-15

SECONDARY ACCESSION(S): ERP000419

REPOSITORIES: ArrayExpress, ENA

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