Dataset Information


Transcription profiling by array of Pichia pastoria strains with different gene dosage of Rhizopus oryzae lipase (Rol) as a model protein

ABSTRACT: Pichia pastoris is a widely used yeast platform for heterologous protein production. Although increasing gene dosage is a powerful strategy to improve recombinant protein production, an excess in the number of gene copies often leads to decreased product yields and increased metabolic burden. In order to find the bottlenecks of this strategy, here we present a transcriptome profile comparison of a series of Pichia pastoris strains carrying a different gene dosage of the Rhizopus oryzae lipase (Rol) as a model protein. Cells were grown on a mixture of glycerol:methanol in chemostat cultures. 2 color experiment in reference design.

ORGANISM(S): Komagataella pastoris  

SUBMITTER: Elena Camara Rey   Pau Ferrer   Joan Albiol  

PROVIDER: E-MTAB-4582 | ArrayExpress | 2017-02-22


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