Transcriptomics

Dataset Information

5

Gene expression analysis in proximal and distal colons of Lcn2-/- IL10-/- double knock-out mice.


ABSTRACT: Lcn2 is involved in host defense against pathogens, but the function in intestinal mucosal immunity and inflammation remains largely unknown. Genetic ablation of Lcn2 results in early-onset colitis and spontaneous emergence of right-sided colonic tumors in the setting of IL-10 deficiency (Lcn2-/-;IL10-/- mice). To address whether inflammation or other mechanisms drives the site-specific tumor locations gene expression analyses in proximal versus distal colons of Lcn2-/- IL10-/- mice were performed. Differential expression between distal colon versus cecum and proximal colon samples were analyses using Affymetrix MoGene 2.0 ST arrays on formalin-fixed, paraffin-embedded tissue sections of Lcn2-/-; IL10-/-mice.

INSTRUMENT(S): GeneTitan Multi-Channel (MC) Instrument

ORGANISM(S): Mus musculus  

SUBMITTER: Romana Gerner   Herbert Tilg   Hubert Hackl   Alexander Moschen  

PROVIDER: E-MTAB-4583 | ArrayExpress | 2016-03-30

REPOSITORIES: ArrayExpress

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Publications


High mucosal and fecal concentrations of the antimicrobial siderophore-binding peptide Lipocalin-2 (Lcn2) are observed in inflammatory bowel disease. However, Lcn2 function in chronic intestinal inflammation remains unclear. Here, we demonstrate that Lcn2 protects from early-onset colitis and spontaneous emergence of right-sided colonic tumors resulting from IL-10 deficiency. Exacerbated inflammation in Lcn2(-/-)/Il10(-/-) mice is driven by IL-6, which also controls tumorigenesis. Lcn2(-/-)/Il10  ...[more]

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