Dataset Information


BiTS Chromatin Immunoprecipitation of H3K4me3 and H3K27ac in Drosophila melanogaster differentiating cardiomyocytes

ABSTRACT: Here we improved BiTS-ChIP (Bonn et al, Nature Protocols 7, 978-994 (2012)) to identify active enhancer and promoter elements genome wide in the 104 cardiomyocytes that constitute the Drosophila heart tube and represents only ~0.5% of the total cell content of the embryo. A transgenic Drosophila strain expressing nuclear GFP under the control of a cardiac specific enhancer (TinC*>GFP) was used for staged embryo collections at stages 13-14 (10-13h of development). After embryo fixation and dissociation, intact fixed nuclei were fluorescent labelling. Purification of this rare nuclear population was achieved by a two-step sorting procedure, yielding ~98% purity. Chromatin was extracted and used for immunoprecipitation and sequencing (ChIP-seq) to analyze chromatin modifications at promoters (H3K4me3 and H3K27ac) and enhancers (H3K27ac). Two independent biological replicates (from FACS sorting, chromatin preparations and ChIP-Seq) were performed for each mark and sequenced using Illumina HiSeq.

INSTRUMENT(S): Illumina HiSeq2000

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Denis Seyres   Charles Girardot  

PROVIDER: E-MTAB-4584 | ArrayExpress | 2017-01-10



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Identification and in silico modeling of enhancers reveals new features of the cardiac differentiation network.

Seyres Denis D   Ghavi-Helm Yad Y   Junion Guillaume G   Taghli-Lamallem Ouarda O   Guichard Céline C   Röder Laurence L   Girardot Charles C   Furlong Eileen E M EE   Perrin Laurent L  

Development (Cambridge, England) 20161201 23

Developmental patterning and tissue formation are regulated through complex gene regulatory networks (GRNs) driven through the action of transcription factors (TFs) converging on enhancer elements. Here, as a point of entry to dissect the poorly defined GRN underlying cardiomyocyte differentiation, we apply an integrated approach to identify active enhancers and TFs involved in Drosophila heart development. The Drosophila heart consists of 104 cardiomyocytes, representing less than 0.5% of all c  ...[more]

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