Dataset Information


Transcription profiling by high throughput sequencing of leaves of resistant (RG-PtoR) tomato plants treated with different Pst DC3000 mutant strains

ABSTRACT: We designed an experiment to assess ETI and PTI in RG-PtoR using a series of Pst DC3000 strains which have different mutations that allow dissection of the plant immune response. We collected samples at 6 hai and monitored the development of disease in these plants. Plants infiltrated with DC3000 ΔfliC had no disease symptoms due to the recognition of AvrPto and AvrPtoB effectors by Pto. In contrast, when these two effectors were absent, the plants developed speck disease (DC3000 ΔavrPto ΔavrPtoB and DC3000 ΔavrPto ΔavrPtoB ΔfliC strains). Plants infiltrated with the triple mutant showed the greatest disease severity due to the absence of ETI and flagellin-activated PTI induction. This experimental design allowed us to identify gene expression changes associated with flagellin-activated PTI (DC3000 ΔavrPto ΔavrPtoB versus DC3000 ΔavrPto ΔavrPtoB ΔfliC) and Pto/Prf-mediated ETI (DC3000 ΔfliC versus DC3000 ΔavrPto ΔavrPtoB ΔfliC). NOTE: Samples in SRA were assigned the same sample accession. This is incorrect as there are different samples, hence 'Source Name' was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.



INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Solanum Lycopersicum

SUBMITTER: Marina Pombo   Greg Martin  

PROVIDER: E-MTAB-4797 | ArrayExpress | 2014-11-04


Dataset's files

Action DRS
E-MTAB-4797.idf.txt Idf
E-MTAB-4797.sdrf.txt Txt
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Transcriptomic analysis reveals tomato genes whose expression is induced specifically during effector-triggered immunity and identifies the Epk1 protein kinase which is required for the host response to three bacterial effector proteins.

Pombo Marina A MA   Zheng Yi Y   Fernandez-Pozo Noe N   Dunham Diane M DM   Fei Zhangjun Z   Martin Gregory B GB  

Genome biology 20140101 10

<h4>Background</h4>Plants have two related immune systems to defend themselves against pathogen attack. Initially,pattern-triggered immunity is activated upon recognition of microbe-associated molecular patterns by pattern recognition receptors. Pathogenic bacteria deliver effector proteins into the plant cell that interfere with this immune response and promote disease. However, some plants express resistance proteins that detect the presence of specific effectors leading to a robust defense re  ...[more]

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