Dataset Information


Transcription profiling by high throughput sequencing of mouse embryonic stem cells following Trim24 and/or p53 knockdown

ABSTRACT: Differentially expressed genes were determined by single-end sequencing (stranded protocol) following Trim24 and/or p53 knock down in the mouse ES cells grown in 2i media. Triplicates were generated for treatment and control samples but for p53 knock down (duplicate).

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus  

SUBMITTER: Mahmoud-Reza Rafiee   Charles Girardot  

PROVIDER: E-MTAB-4893 | ArrayExpress | 2017-11-08



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Expanding the Circuitry of Pluripotency by Selective Isolation of Chromatin-Associated Proteins.

Rafiee Mahmoud-Reza MR   Girardot Charles C   Sigismondo Gianluca G   Krijgsveld Jeroen J  

Molecular cell 20161020 3

Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog (OSN), yet a systematic investigation of the composition and dynamics of the OSN protein network specifically on chromatin is still missing. Here we have developed a method combining ChIP with selective isolation of chromatin-associated proteins (SICAP) followed by mass spectrometry to identify chromatin-bound partners of a protein of interest. ChIP-SICAP in mouse embryonic stem ce  ...[more]

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