Transcriptomics

Dataset Information

0

Transcription profiling of HL-60 acute myeloid leukemia cells treated with Daunorubicin (DNR), Cytarabin (Ara-C), or NADPH oxidase inhibitor VAS2870


ABSTRACT: Daunorubicin (DNR) and Cytarabin (Ara-C) are the main chemotherapeutic drugs used for Acute Myeloid Leukemias (AML) treatment. However, their mode of action is not well understood. To decipher if these drug would induce rapid transcriptional reprogramming, we treated HL-60 AML cell line with DNR or Ara-C for 3 hours and carried out a transcriptomic analyses. In addition, we had shown that Reactive Oxigen Species (ROS) produced by NADPH oxidases (NOX) are involved in DNR-induced gene expression. We therefore also performed a transcriptomic analyses in HL-60 cells treated with DNR and VAS2870, an NADPH oxidase inhibitor. HL-60 cells were treated or not for 3 hours with 2 µM Ara-C, 1 µM DNR or 1 µM DNR + 20µM VAS 2870. RNAs were purified from 3 independent experiments and used to probe Affymetrix Human Gene 2.0 ST Genechips

ORGANISM(S): Homo sapiens  

DISEASE(S): Acute Myeloid Leukemia

SUBMITTER: Tony KAOMA  

PROVIDER: E-MTAB-4895 | ArrayExpress | 2017-12-31

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-MTAB-4895.idf.txt Idf
E-MTAB-4895.processed.1.zip Processed
E-MTAB-4895.raw.1.zip Raw
E-MTAB-4895.sdrf.txt Txt
Items per page:
1 - 4 of 4

Similar Datasets

2022-01-08 | GSE193094 | GEO
2016-07-04 | E-GEOD-52919 | ArrayExpress
2016-01-01 | S-EPMC7115946 | BioStudies
1000-01-01 | S-EPMC5292678 | BioStudies
2017-01-01 | S-EPMC5432104 | BioStudies
1969-12-31 | S-SCDT-EMM-2019-10419 | BioStudies
2020-01-01 | S-EPMC7552348 | BioStudies
2016-01-01 | S-EPMC5457273 | BioStudies
2020-01-01 | S-EPMC7718665 | BioStudies
2020-01-01 | S-EPMC7569086 | BioStudies