Proteomics

Dataset Information

43

Identification of proteins interacting with lncRNA Braveheart in mouse using protein microarray


ABSTRACT: The mouse lncRNA Braveheart (Bvht) as a non-coding transcript has been found to act in trans to regulate cardiovascular lineage commitment. However, the mechanism of Bvht action is still not clear. lncRNAs have been shown to regulate gene expression though cooperating with protein partners. Recently, we experimentally determine the secondary structure of Bvht containing a novel structural motif AGIL. AGIL motif deletion (BvhtdAGIL) in mouse embryonic stem cells prevents the transition from mesoderm cells to cardiac progenitors. To identify proteins that interact with the Bvht AGIL motif, we used a human protein microarray platform (Human ProtoArray, Life Technology). Full-length Bvht and BvhtdAGIL transcripts were generated by in vitro transcription and labeled with Cy5. 50pmol Cy5-labeled RNAs were individually incubated with the protein microarray.

ORGANISM(S): Mus musculus  

SUBMITTER: Zhihong Xue  

PROVIDER: E-MTAB-4995 | ArrayExpress | 2016-09-26

REPOSITORIES: ArrayExpress

Similar Datasets

2011-07-31 | E-GEOD-28881 | ArrayExpress
2014-05-15 | E-GEOD-50629 | ArrayExpress
2015-11-23 | E-GEOD-51817 | ArrayExpress
2017-10-01 | E-MTAB-5369 | ArrayExpress
| GSE77752 | GEO
2015-12-16 | E-GEOD-59915 | ArrayExpress
2012-10-10 | E-GEOD-34794 | ArrayExpress
2010-06-25 | E-GEOD-4460 | ArrayExpress
2016-07-17 | E-GEOD-83442 | ArrayExpress
2012-12-03 | E-GEOD-42673 | ArrayExpress