Transcriptomics

Dataset Information

297

RNA-seq analysis of Helicobacter cinaedi ATCC BAA-847 under microaerobic and aerobic culture conditions


ABSTRACT: Helicobacter cinaedi is an emerging bacterial pathogen of immunosuppressed individuals. The species is traditionally thought to require an H2-enhanced microaerobic atmosphere for growth, although it can proliferate under aerobic conditions when co-cultured with epithelial monolayers or supplemented with certain metabolites (notably, L-lactate). The goal of this experiment was to assess the global transcription changes that occur in the H. cinaedi type strain (ATCC BAA-847) under various media and atmospheric conditions. These include bacterial monoculture, as well as co-culture with Caco-2 intestinal epithelial cells. In total, Illumina mRNA-seq (stranded, paired-end) was performed on H. cinaedi grown under 9 in vitro culture conditions (4-5 biologic replicates per condition).

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Helicobacter cinaedi CCUG 18818 = ATCC BAA-847  

SUBMITTER: Jonathan E Schmitz   Timothy L Cover  

PROVIDER: E-MTAB-5062 | ArrayExpress | 2016-09-19

SECONDARY ACCESSION(S): ERP016954

REPOSITORIES: ArrayExpress, ENA

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Publications

Epithelial Coculture and l-Lactate Promote Growth of Helicobacter cinaedi under H2-Free Aerobic Conditions.

Schmitz Jonathan E JE   Taniguchi Takako T   Misawa Naoaki N   Cover Timothy L TL  

Applied and environmental microbiology 20161027 22


Helicobacter cinaedi is an emerging opportunistic pathogen associated with infections of diverse anatomic sites. Nevertheless, the species demonstrates fastidious axenic growth; it has been described as requiring a microaerobic atmosphere, along with a strong preference for supplemental H2 gas. In this context, we examined the hypothesis that in vitro growth of H. cinaedi could be enhanced by coculture with human epithelial cells. When inoculated (in Ham's F12 medium) over Caco-2 monolayers, the  ...[more]

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