Transcriptomics

Dataset Information

60

Transcriptional response of LPS-stimulated monocyte-derived macrophages in the presence or absence of the anti-TNF agonist Etanercept


ABSTRACT: We measured TNFα activity on the transcriptional level by identifying TNFα-inducible genes in human blood monocyte-derived macrophages (MDM). On the basis that TNFα does not act in isolation, we used bacterial lipopolysaccharide (LPS) as a prototypic model of a stimulus to invoke secretion of a wide range of immunologically active factors, including TNFα. In order to identify gene expression attributable to LPS-induced TNFα, we compared the transcriptome of MDM 24 hours after stimulation with 100 ng/ml ultra-pure LPS in the presence and absence of the soluble TNF receptor fusion protein Etanercept (10 µg/ml).

ORGANISM(S): Homo sapiens  

SUBMITTER: Carolin Turner  

PROVIDER: E-MTAB-5095 | ArrayExpress | 2017-08-04

REPOSITORIES: ArrayExpress

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Publications

Tumor Necrosis Factor (TNF) Bioactivity at the Site of an Acute Cell-Mediated Immune Response Is Preserved in Rheumatoid Arthritis Patients Responding to Anti-TNF Therapy.

Byng-Maddick Rachel R   Turner Carolin T CT   Pollara Gabriele G   Ellis Matthew M   Guppy Naomi J NJ   Bell Lucy C K LCK   Ehrenstein Michael R MR   Noursadeghi Mahdad M  

Frontiers in immunology 20170804


The impact of anti-tumor necrosis factor (TNF) therapies on inducible TNF-dependent activity in humans has never been evaluated in vivo. We aimed to test the hypothesis that patients responding to anti-TNF treatments exhibit attenuated TNF-dependent immune responses at the site of an immune challenge. We developed and validated four context-specific TNF-inducible transcriptional signatures to quantify TNF bioactivity in transcriptomic data. In anti-TNF treated rheumatoid arthritis (RA) patients,  ...[more]

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