Genomics

Dataset Information

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ChIP-seq based genome wide profiling of the histone mark H3K27ac and transcription factor Nuclear Factor 1 (NFI) in a differentiating murine brown preadipocyte cell line (IBA)


ABSTRACT: ChIP sequencing was used to generate genome-wide maps of the histone mark H3K27ac during in vitro differentiation of a murine brown preadipocyte cell line (IBA) at five different time points, in two biological replicates: Day 0 (confluence), 2h (post induction), Day 1, Day 2 and Day 4 (mature brown adipocytes). Additionally, transcription factor (TF) localisation maps of the Nuclear Factor 1 (NFI) were generated using ChIP sequencing at two time points: Day 0 and Day 4.

INSTRUMENT(S): NextSeq 550, Illumina HiSeq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: Rachana Pradhan  

PROVIDER: E-MTAB-5159 | ArrayExpress | 2017-09-21

SECONDARY ACCESSION(S): ERP104274

REPOSITORIES: ArrayExpress, ENA

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Publications

Dissecting the brown adipogenic regulatory network using integrative genomics.

Pradhan Rachana N RN   Bues Johannes J JJ   Gardeux Vincent V   Schwalie Petra C PC   Alpern Daniel D   Chen Wanze W   Russeil Julie J   Raghav Sunil K SK   Deplancke Bart B  

Scientific reports 20170209


Brown adipocytes regulate energy expenditure via mitochondrial uncoupling, which makes them attractive therapeutic targets to tackle obesity. However, the regulatory mechanisms underlying brown adipogenesis are still poorly understood. To address this, we profiled the transcriptome and chromatin state during mouse brown fat cell differentiation, revealing extensive gene expression changes and chromatin remodeling, especially during the first day post-differentiation. To identify putatively causa  ...[more]

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