Transcriptomics

Dataset Information

337

MRNA-seq based gene expression profiling of differentiating murine brown preadipocytes (IBA)


ABSTRACT: mRNA-sequencing was used to profile temporal changes in gene expression during in vitro differentiation of IBA at five different time points: Day 0 (confluence), 2h (post induction), Day 1, Day 2 and Day 4 (mature brown adipocytes), performed in two biological replicates (with two technical replicates in the first biological replicate).

INSTRUMENT(S): NextSeq 550, Illumina HiSeq 2000

ORGANISM(S): Mus musculus  

SUBMITTER: Rachana N Pradhan  

PROVIDER: E-MTAB-5160 | ArrayExpress | 2017-10-14

SECONDARY ACCESSION(S): ERP104606

REPOSITORIES: ArrayExpress, ENA

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Publications

Dissecting the brown adipogenic regulatory network using integrative genomics.

Pradhan Rachana N RN   Bues Johannes J JJ   Gardeux Vincent V   Schwalie Petra C PC   Alpern Daniel D   Chen Wanze W   Russeil Julie J   Raghav Sunil K SK   Deplancke Bart B  

Scientific reports 20170209


Brown adipocytes regulate energy expenditure via mitochondrial uncoupling, which makes them attractive therapeutic targets to tackle obesity. However, the regulatory mechanisms underlying brown adipogenesis are still poorly understood. To address this, we profiled the transcriptome and chromatin state during mouse brown fat cell differentiation, revealing extensive gene expression changes and chromatin remodeling, especially during the first day post-differentiation. To identify putatively causa  ...[more]

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