Transcriptomics

Dataset Information

77

Antibody repertoire RNA-seq throughout key stages of mouse B-cell development (pre-B-cells, naive B cells, plasma cells)


ABSTRACT: We sought to investigate the extent of genetic and antigen-driven predetermination of antibody repertoires throughout B-cell development (pre-B cells, naive B cells, plasma cells). To this end, we used (i) an inbred model organism (mouse, C57BL/6J) to quantify genetic predetermination and (ii) cohort-stratification (4 cohorts: 1 untreated, 3 antigen-immunized) to quantify antigen-driven predetermination. To ensure generalizability of our research, we additionally sequenced naive B cells from Balb/c and pet shop mice.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Mus musculus  

SUBMITTER: Victor Greiff  

PROVIDER: E-MTAB-5349 | ArrayExpress | 2017-03-28

SECONDARY ACCESSION(S): ERP020577

REPOSITORIES: ArrayExpress, ENA

Dataset's files

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E-MTAB-5349.idf.txt Idf
E-MTAB-5349.idf.txt_original Idf
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Publications

Comprehensive evaluation and optimization of amplicon library preparation methods for high-throughput antibody sequencing.

Menzel Ulrike U   Greiff Victor V   Khan Tarik A TA   Haessler Ulrike U   Hellmann Ina I   Friedensohn Simon S   Cook Skylar C SC   Pogson Mark M   Reddy Sai T ST  

PloS one 20140508 5


High-throughput sequencing (HTS) of antibody repertoire libraries has become a powerful tool in the field of systems immunology. However, numerous sources of bias in HTS workflows may affect the obtained antibody repertoire data. A crucial step in antibody library preparation is the addition of short platform-specific nucleotide adapter sequences. As of yet, the impact of the method of adapter addition on experimental library preparation and the resulting antibody repertoire HTS datasets has not  ...[more]

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