Dataset Information


Transcription profiling by array of rat primary hepatic stellate cells with varying degrees of in vitro activation

ABSTRACT: Gene expression was determined in primary rat hepatic stellate cells during the in vitro activation process in freshly isolated (4h), quiescent (1d), early activated (3d) and fully activated (7d) hepatic stellate cells. The cells were isolated from the liver using density centrifugation and cultured on plastic in DMEM containing serum for the indicated time. RNA was isolated using the Qiagen Rneasy Mini Kit. The Affymetrix Gene Chip Rat Gene 2.0 ST was used for gene expression analysis performed by the genomic core facility of the EMBL (Heidelberg, Germany). All experiments were performed three times with independent animals.

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Eva C Schumacher  

PROVIDER: E-MTAB-5404 | ArrayExpress | 2017-10-23


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Combined Methylome and Transcriptome Analysis During Rat Hepatic Stellate Cell Activation.

Schumacher Eva Christine EC   Götze Silke S   Kordes Claus C   Benes Vladimir V   Häussinger Dieter D  

Stem cells and development 20171128 24

Hepatic stellate cells (HSCs) are mesenchymal stem cells (MSCs) of the liver. They are unique among MSCs, since HSCs remain in a quiescent, retinoid-storing state in the normal liver but become activated after liver injury and contribute to tissue repair. The epigenetic mechanisms accompanying the transition of HSCs from a quiescent to an activated state are in the focus of the present study. We investigated the methylome and transcriptome during this process and observed profound changes. While  ...[more]

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