Genomics

Dataset Information

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ChIP-seq of the multiple antibiotic resistance activator (MarA) protein and RNA polymerase sigma70 subunit of Enterotoxigenic Escherichia coli strain H10407


ABSTRACT: The experiment contains ChIP-seq data for Enterotoxigenic Escherichia coli strain H10407 transformed with plasmid pRGM9818. The strain was grown at 37 degrees in LB medium and crosslinked with 1 % (v/v) formaldehyde. After sonication, to break open cells and fragment DNA, immunoprecipitations were done using antibodies against MarA, MarR or the RNA polymerase sigma 70 subunit. Libraries were prepared using DNA remaining after immunoprecipitation. Note that the MarR immunoprecipitations were not successful but the data served as a useful control for non-specifically immunoprecipitated DNA sequences.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Escherichia Coli

SUBMITTER: David C Grainger  

PROVIDER: E-MTAB-5521 | ArrayExpress | 2017-09-30

SECONDARY ACCESSION(S): ERP021743

REPOSITORIES: ArrayExpress, ENA

Dataset's files

Source:
Action DRS
E-MTAB-5521.idf.txt Idf
E-MTAB-5521.idf.txt_original Idf
E-MTAB-5521.sdrf.txt Txt
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