Transcriptomics

Dataset Information

341

Stromal myofibroblasts drive tumorigenesis in Peutz-Jeghers Syndrome polyposis models


ABSTRACT: Germline mutations in LKB1 predispose to hereditary Peutz-Jeghers Syndrome (PJS), manifesting with gastrointestinal polyposis. We discovered that conditional deletion of Lkb1 in stromal fibroblasts using Fsp1-Cre leads to expansion of stromal cells and gastrointestinal polyposis in mice. Here we have investigated gene expression signatures in the Fsp1-Cre;Lkb1fl/fl mouse polyps harbouring bi-allelic deletion of Lkb1 in stromal cells together with wild-type epithelium. We provide RNA-seq gene expression data of 6 polyps, 4 adjacent gastric mucosa samples and 5 wild-type gastric mucosa samples from littermate controls. Our experiment demonstrates e.g. activated cytokine signaling and inflammatory pathways in the polyps.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus  

SUBMITTER: Saara Ollila   Eva Domnech-Moreno   Tomi P Mkel  

PROVIDER: E-MTAB-5549 | ArrayExpress | 2018-01-05

SECONDARY ACCESSION(S): ERP021891

REPOSITORIES: ArrayExpress, ENA

altmetric image

Publications


Germline mutations in the gene encoding tumor suppressor kinase LKB1 lead to gastrointestinal tumorigenesis in Peutz-Jeghers syndrome (PJS) patients and mouse models; however, the cell types and signaling pathways underlying tumor formation are unknown. Here, we demonstrated that mesenchymal progenitor- or stromal fibroblast-specific deletion of Lkb1 results in fully penetrant polyposis in mice. Lineage tracing and immunohistochemical analyses revealed clonal expansion of Lkb1-deficient myofibro  ...[more]

Similar Datasets

2017-01-23 | E-MTAB-5255 | ArrayExpress
| GSE73547 | GEO
2009-09-21 | E-TABM-643 | ArrayExpress
2010-10-06 | E-MEXP-2444 | ArrayExpress
| phs001935 | dbGaP
2015-04-29 | E-GEOD-62518 | ArrayExpress
2010-12-01 | GSE19963 | GEO
2010-12-01 | E-GEOD-19963 | ArrayExpress
2007-08-28 | E-GEOD-6135 | ArrayExpress
| PRJEB19821 | ENA