Browse
Submit Data
Database
Help
Login

Dataset Information

1748 Views

26 Connections

0 Citations

0 Reanalyses

3 Downloads

Omics score: 620

RNA-IP of TAP-tagged ZC3H32 in bloodstream-form Trypanosoma brucei

ABSTRACT: TAP RNA-IP - ZC3H32 (Tb927.10.5250)

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Christine Clayton

PROVIDER: E-MTAB-5612 | ArrayExpress | 2017-12-06

SECONDARY ACCESSION(S): ERP105576

REPOSITORIES: ArrayExpress, ENA

ACCESS DATA

Json Xml

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

RNA-IP of TAP-tagged 4EIP in Trypanosoma brucei

Project description:TAP RNA-IP - 4EIP (Tb927.9.11050)

2017-12-06 | E-MTAB-6240 | ArrayExpress

TAP RNA-IP - ZC3H22

Project description:TAP RNA-IP - ZC3H22 (Tb927.7.2680)

2018-06-18 | E-MTAB-6906 | ArrayExpress

TAP RNA-IP - MKT1L

Project description:TAP RNA-IP - MKT1L (Tb927.10.1490)

2018-06-18 | E-MTAB-6904 | ArrayExpress

TAP RNA-IP - MKT1

Project description:TAP RNA-IP - MKT1 (Tb927.6.4770)

2018-06-18 | E-MTAB-6907 | ArrayExpress

RNA-IP of TAP-tagged ZC3H32 in bloodstream-form Trypanosoma brucei

Project description:RNA-IP of TAP-tagged ZC3H32 in bloodstream-form Trypanosoma brucei

| PRJEB23798 | ENA

RNAi of XAC1 (Tb927.7.2780) in Trypanosoma brucei bloodstream forms

Project description:Gradient fractions of RNAi of XAC1 (Tb927.7.2780) in Trypanosoma brucei bloodstream forms. RNAi was induced using tetracycline and cell extracts were fractionated into polysomal and monosome-non-ribosome-associated fractions.

2017-12-06 | E-MTAB-6239 | ArrayExpress

RNA-Seq data: RNA-IP (TAP-ZC3H30 pull down followed by RNA-Seq) and total transcriptomics (Effect of ZC3H30 gene deletion) in procyclic form of T. brucei

Project description:The first 4 samples belong to the RNA-IP using in situ TAP tagged ZC3H30 in procyclic (insect) form of the parasite T. brucei Lister 427, 2 samples are Elu or eluate, and 2 are FL or flowthrough (unbound) sample. The other 8 samples are also from procyclic cells. 4 samples belong to DKO(ZC3H30 gene double knockout), 2 are non-stressed and 2 are heat shocked samples; the rest 4 samples are DKO-ectopic (ZC3H30 double knockouts, expressing, ectopic copy of ZC3H30) 2 are non-stressed and 2 are heat shocked samples. Heat Shock experiment was done at 39 degree Celsius.

2017-12-06 | E-MTAB-6281 | ArrayExpress

Transcriptome sequencing of bloodstream form Trypanosoma brucei expressing VSG121

Project description:Cells were grown in HMI-9 and RNA prepared using QIAGEN RNAeasy kit.

2017-02-12 | E-MTAB-5460 | ArrayExpress

RNA-IP of TAP-tagged 4EIP in Trypanosoma brucei

Project description:RNA-IP of TAP-tagged 4EIP in Trypanosoma brucei

| PRJEB23807 | ENA

Continuous culture of bloodstream form and procyclic form of Trypanosoma brucei

Project description:Trypanosomes are a globally important group of parasites which together kill and debilitate millions of people world-wide. In trypanosomes, genes do not have individual promoters, rather ~10000 genes share ~200 promoters and all gene expression is thus regulated post-transcriptionally. While effector proteins which modulate the expression of many genes have been described, the mechanisms by which trypanosomes sense changes in their environment and manifest changes in gene expression remain elusive. This study demonstrates that trypanosomes sense changes in their environment through temperature sensitive RNA secondary structure. We show that the majority of observed mRNA abundance changes which distinguish insect adapted and bloodstream adapted life cycle stages can be explained through change in temperature alone.

2016-06-14 | E-MTAB-3335 | ArrayExpress

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
GPMDB

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS

ArrayExpress
ExpressionAtlas

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data